Tetracyclines are a class of broad-spectrum antibiotics produced by actinomycetes. They are widely used in infections caused by various bacteria, rickettsia, chlamydia, mycoplasma, etc. In vivo, Tetracyclines complexes formed by calcium ions are yellow and deposited on bones and teeth.
Children taking Tetracyclines will have yellow teeth, and pregnant women may have discoloration of teeth and bone growth inhibition after taking Tetracyclines. Therefore, children and pregnant women should be careful with this drug or take it.
1. Test basis
This scheme is based on the standard of GB T 24800.1-2009 High performance liquid chromatography for the determination of nine tetracycline antibiotics in cosmetics.
2. Detection indicators
MINO, oxytetracycline (OTC), tetracycline (TC), differential tetracycline (ETC), aureomycin (CTC), differential aureomycin (MCTC) ), dehydrated tetracycline (ATC), dehydrated tetracycline (ETAC), doxycycline (MTC), demethylaureomycin (DMCTC), doxycycline (DC).
3. Instrument preparation
4. Chromatographic conditions
Chromatographic column: C18 column, 4.6 mm*250 mm, particle size 5 μm
Detection wavelength: 350nm
Test temperature: room temperature
The flow speed: 1 ml/min
Mobile phase: A: acetonitrile B: 0.01moL/L oxalic acid solution, gradient elution
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