Before using
1. Mobile phase should choose chromatographic pure reagent, water is heavy distilled water.
2. All incoming mobile phase and sample must be filtered through 0.45 micron membrane and injected during filtration. Meaning to distinguish the use of water membrane and organic membrane.
Meaning to distinguish the use of water membrane and organic membrane;
Meaning to distinguish the use of water membrane and organic membrane;
3.Pay attention to the scope of application, such as PH value and mobile phase type, before using the column. Recommended use of protection column.
4. Ensure good grounding of laboratory power supply and stable voltage.
In the use
1. Ensure that the exhaust valve is open before discharge.
2. If there is no mobile phase in the liquid inlet pipe, the pipeline should be filled with mobile phase before drainage to avoid dry drainage.
3.Avoid switching machine repeatedly in a short time.
4. Timely and regular backup of experimental data.
5. Replace parts in time when leakage occurs to prevent other parts of the instrument from being corroded by the mobile phase.
After using
1. Cleaning salt-containing flow: first rinse with 10% methanol-aqueous solution for 1 hour, and then rinse with pure methanol, wash for 30 minutes; In the next experiment, salt-containing mobile phase was still used, and 10% methanol-water solution was used first, wash for 30 minutes before using the required mobile phase;
2. If the instrument is not used for a long time, run it with pure methanol for 30 minutes every week.
3.The instrument should be placed in a constant temperature, dry, dust-free environment.
4. Do not disassemble the instrument without permission.