Vitamin C, also known as ascorbic acid, is water-soluble vitamin, not only can beauty, but also anti-oxidation, cell protection, and even effective anti-cancer, is the most famous nutrient. In this paper, high performance liquid chromatograph was used to detect the content of vitamin C in fruit juice. The analysis results were rapid and accurate. Vitamin C in fruit juice can be separated on ODS column, and vitamin C can be absorbed at the wavelength of 206nm.
1. Determination of vitamin C content in fruit juice by HPLC
1.1 High performance liquid chromatography: unit pump, variable wavelength UV detector, data analysis system.
1.2 Chromatographic column: 150mm×4.6mm, 5µL particles bonded ODS column.
1.3 Injector: 25µL microinjection needle.
2. Analysis conditions of vitamin C content in fruit juice by HPLC
2.1 Mobile phase: 0.01mol/L HCl solution.
2.2 Flow rate: 1.0ml /min.
2.3 Column temperature: 40.
2.4 Detection wavelength: 206nm.
2.5 Retention time: 3.2min.
3.Determination steps of vitamin C content in fruit juice by HPLC
3.1 Sample preparation: 4.0g of concentrated juice sample was weighed, accurate to 0.01g, dissolved in 50mL volumetric bottle with mobile phase, and the volume was fixed to scale, shaken well, and the sample was filtered into the sample bottle with 0.45µm water filter membrane as sample solution.
3.2 Under the above operating conditions, wait for the stable operation of the testing instrument, and cross test according to the sequence of standard solution and sample solution.