Application Solutions – Changior Instrument

Application of high performance liquid chromatograph in biopharmaceutics

changior — Tue, 26 Apr 2022 04:02:32 +0000

High performance liquid chromatography (HPLC) is an efficient and rapid separation and analysis technology developed in recent decades, and is an important tool for modern separation and detection. Highly effective liquid phase color spectrometer based on classical liquid chromatograph, introducing the theory and experiment method of gas chromatography, the mobile phase instead of high pressure conveying, adopt efficient fixed phase and on-line detection methods, development of separation and analysis techniques, with a high pressure, high speed, high efficiency, high sensitivity, high selectivity and wide scope of application, It has become one of the fastest developing and most widely used modern analytical techniques in the field of biopharmaceutical.

Application of high performance liquid chromatography in the detection of drug content:

Drugs have a great relationship with the improvement of human health and quality of life. Due to the influence of purity, the efficacy of many drugs cannot be fully played, and even produce toxic side effects. Sometimes all kinds of tests need to be done before taking, which brings a lot of trouble. The detection of high performance liquid chromatography is relatively simple, which is the best choice for separating and purifying drugs. Because high performance liquid chromatography has the characteristics of high sensitivity, rapid simplicity and specificity, widely used in drug analysis, especially in the time of more interference performance than other methods.

Application of high performance Liquid chromatography in the detection of pharmaceutical wastewater:

The environmental pollution caused by residual drug components in pharmaceutical wastewater is becoming more and more serious. The treatment of pharmaceutical wastewater has become a very important aspect of environmental protection. High performance liquid chromatograph can simultaneously determine the content of crosssalamycin, theophylline and paracetamol in pharmaceutical wastewater, through the detection of pharmaceutical wastewater, to find a suitable way to remove harmful substances in pharmaceutical wastewater.

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Solution: Liquor composition analysis

changior — Wed, 30 Mar 2022 10:12:01 +0000

Chinese liquor culture has a long history, extensive and profound. Aroma components of liquor include alcohols, esters, acids, aldehydes and ketones, acetals, aromatic compounds, nitrogenous compounds and furan compounds, etc. Esters are aromatic compounds, which play an important role in all kinds of flavor liquor and are the main factor of forming strong aroma of liquor.

Ethyl acetate, ethyl propionate and ethyl lactate were important flavor components in liquor. The detection and control of various components in the process of liquor (flavor) production is the key to ensure the quality of liquor (flavor). More and more brand liquor production enterprises use scientific gas chromatography analysis for quality control and development of new products, and technical supervision departments at all levels use gas chromatography to monitor the quality of liquor.

1.Detection Index

Ethyl lactate, ethyl caproate, ethyl acetate, methanol, etc

2.Instrument configuration

1．Gas chromatograph with hydrogen flame ionization detector, high sensitivity
2．Chromatographic column: capillary liquor column 30m*0.32mm*0.5um
3．Standard product: 22 components of liquor standard sample
4．Chromatography workstation: double channel, a workstation can be connected to two chromatographs
5．Gas source: nitrogen cylinder gas (99.999%), hydrogen generator, air generator

3.Experimental conditions

1． Column temperature: initial temperature 50℃ initial 5min, 6℃/min, final temperature 160℃, final 10min
2． Carrier gas: nitrogen
3． Column pressure: 0.05mpa
4． Fractional injection
5． Vaporization: 230℃
6． Detector: FID 250℃

4.Spectra data

5.Suite of Equipment

Determination of ethylene oxide (EO) residues in medical devices

changior — Wed, 30 Mar 2022 09:45:27 +0000

Ethylene oxide (EO) is an organic compound with the chemical formula C2H4O. It is a known carcinogen and is often used as a sterilizer. Relevant regulations require regular testing in production and use. Ethylene oxide residue According to GB/T 16886.7-2015 biological Evaluation of Medical Devices part 7: Ethylene oxide residue sterilization GB/T 14233.1-2008 Medical infusion, blood transfusion, injection equipment test method Part 1: Chemical Analysis Methods, GB19083-2010 Technical Requirements for Medical Protective Masks, ISO10993-7; 2008 “ethylene oxide (EO) and 2-chloroethanol (ECH) residue detection” in the gas chromatography for detection.

Our company’s ethylene oxide (EO) detection solution is to quantitatively determine the content of EO in the sterilized medical equipment with headspace air chromatograph. The instrument configuration of this solution is reasonable. Simple operation is a practical testing scheme for medical equipment manufacturers.

1.Detection index

ethylene oxide (EO)

2.Reagent

Ethylene oxide (EO) standard

3.Experimental conditions

Gas phase detection conditions: column temperature 60℃, vaporization chamber 220℃, FID detector 250℃
Headspace sampling conditions: sample heating temperature 60℃, valve box temperature 100℃, pipeline temperature 120℃

4.Spectra data

Headspace injection volume was 1mL, and internal standard method was used for analysis.

5.Instrument Configuration

Determination of total organic volatile organic compounds (TVOC) in indoor air

changior — Tue, 29 Mar 2022 08:03:20 +0000

Application of Tenax TA adsorption tubes to collect a certain volume of air samples, the volatile organic compounds in air flow holds in the adsorption, after sampling, is obtained by thermal desorption device heating tube adsorption desorption gas of volatile organic compounds under test sample with an inert carrier gas into the capillary gas chromatograph, with the retention time qualitative and quantitative peak height or peak area.

1.Test basis

This protocol refers to GB 50325-2010 Appendix G “Determination of Total volatile organic Compounds (TVOC) in indoor air”.

2.Detection indicators

TVOC

3.Instrument preparation

4.Experimental conditions

Inlet temperature: 220℃
Column temperature: initial temperature 50℃, initial 5min, heating rate 6℃/min, final temperature 250℃, final 5min
Detector temperature: 250℃
Carrier gas: nitrogen, flow rate is about 30 mL /min, pre-column pressure is 0.06Mpa; Shunt sample
Combustion gas: hydrogen, flow rate of about 30 mL /min
Auxiliary gas: air, flow rate is about 300ml/m

5.Atlas data

Automobile interior inspection scheme

changior — Tue, 29 Mar 2022 07:47:44 +0000

The headspace method uses a headspace sampler and gas chromatograph. The sampling method is relatively simple, and the sampling is completed in the headspace sampler. 2 grams of samples were taken from the test sample and put into a 20ml headspace bottle, which was kept warm and balanced at 120℃ for 60min. Then a certain amount of gas was extracted from the headspace bottle with a syringe, and analyzed by gas chromatograph and FID detector.

1.Test basis

This scheme is based on THE European standard VDA277 in ISO/TS16949, and is also a common method for measuring benzene hydrocarbon volatile organic compounds in European automotive enterprises.

2.Detection indicators

Nonmetallic materials in automotive interior include benzene, toluene, xylene, ethylbenzene, styrene, formaldehyde, tetradecane, etc.

3.Instrument preparation

4.Experimental conditions

Inlet temperature: 200℃
Column temperature: initial temperature 50℃, initial temperature 5min, heating rate 12℃/min, final temperature 200℃, final temperature 3min
Detector temperature: 250℃
Carrier gas: helium, flow rate is about 30 mL /min
Combustion gas: hydrogen, flow rate of about 30 mL /min
Auxiliary gas: air, flow rate is about 300ml/min

5.Atlas data

Determination of solvent residue in edible vegetable oil

changior — Mon, 28 Mar 2022 09:04:31 +0000

Three main hexaalkanes other than n-hexane in the solvent residue of edible vegetable oil were determined by mass spectrometry. With n-hexane as the standard material, the solvent residue in edible vegetable oil was determined by headspace injection gas chromatography. The qualitative and quantitative determination methods of solvent residues in edible vegetable oil were studied.

1. Test basis

The scheme is prepared according to GB 5009.262-2016 national food safety standard Determination of solvent residues in food.

2. Test indicators

N-hexane

3. Instrument preparation

4. Experimental conditions

4.1 Headspace analysis conditions
Balance temperature: 50℃, quantitative ring temperature: 60℃, transmission temperature: 70℃, cycle time: 10min, balancing time: 30min, pressurizing time: 0.05min, quantitative ring filling time: 0.15min, quantitative ring balancing time: 0.10min, injection time: 1.00min, injection volume: 1mL.

4.2 Gas chromatographic analysis conditions
Column temperature 180℃, injector temperature: 200℃, flame ionization (FID) detector, detector temperature: 250℃, hp-5 (30m×0.25mm×0.25μm) elastic quartz capillary column, high purity nitrogen gas, flow rate of 4.4mL/min, hydrogen 50mL/min, air flow rate of 450mL/min, tail air: 15mL/min, split ratio: 1∶1.

5. Atlas data

Detection scheme of antioxidant BHA, BHT, PG, TBHQ in food

changior — Mon, 28 Mar 2022 02:16:50 +0000

Terbutylhydroquinone (TBHQ) and dibutylhydroxy toluene (BHT) are antioxidants in food additives, which can prevent or delay the oxidative decomposition and deterioration of oil or food ingredients and improve food stability. However, the addition of antioxidants must comply with the provisions of China’s food hygiene law, excessive addition will damage human health.

1. Test basis

This program is prepared according to “Determination of 9 antioxidants in Food of National Food Safety Standards”.

2. Detection indicators

Tert-butyl-p-hydroxyanisole (BHA), dibutyl hydroxytoluene (BHT), propyl gallate (PG), tert-butylhydroquinone (TBHQ).

3. Instrument preparation

4. Experimental conditions

Chromatographic column: C18 column, 4.6 mm*250 mm, particle size 5 μm
Detection wavelength: 280nm
Test temperature: room temperature
The flow speed: 1 ml/min
The flow phase: 1.5% (volume fraction) acetic acid-methanol solution (A), 1.5% (volume fraction) acetic acid-aqueous solution (B);
Elution gradient: 0min-5min, mobile phase A is 30%, 5min-20min, mobile phase A increases linearly from 30% to 80%, 20min-30min; Mobile phase A is 80%; The flow rate was 1.0mL/min and the column temperature was 40℃

5. Atlas data

Detection protocol for 11 tetracycline antibiotics

changior — Mon, 28 Mar 2022 01:53:46 +0000

Tetracyclines are a class of broad-spectrum antibiotics produced by actinomycetes. They are widely used in infections caused by various bacteria, rickettsia, chlamydia, mycoplasma, etc. In vivo, Tetracyclines complexes formed by calcium ions are yellow and deposited on bones and teeth.

Children taking Tetracyclines will have yellow teeth, and pregnant women may have discoloration of teeth and bone growth inhibition after taking Tetracyclines. Therefore, children and pregnant women should be careful with this drug or take it.

1. Test basis

This scheme is based on the standard of GB T 24800.1-2009 High performance liquid chromatography for the determination of nine tetracycline antibiotics in cosmetics.

2. Detection indicators

MINO, oxytetracycline (OTC), tetracycline (TC), differential tetracycline (ETC), aureomycin (CTC), differential aureomycin (MCTC) ), dehydrated tetracycline (ATC), dehydrated tetracycline (ETAC), doxycycline (MTC), demethylaureomycin (DMCTC), doxycycline (DC).

3. Instrument preparation

4. Chromatographic conditions

Chromatographic column: C18 column, 4.6 mm*250 mm, particle size 5 μm
Detection wavelength: 350nm
Test temperature: room temperature
The flow speed: 1 ml/min
Mobile phase: A: acetonitrile B: 0.01moL/L oxalic acid solution, gradient elution

Testing method for chemical solvent products

changior — Sun, 27 Mar 2022 03:29:15 +0000

Paint is a material that can be coated on the surface of an object with different construction processes to form a solid film of strong adhesion and continuous strength. The film formed in this way is commonly known as coating film, also known as paint film or coating.

Chemical raw materials can generally be divided into organic chemical raw materials and inorganic chemical raw materials: Organic chemical raw materials can be divided into alkanes and their derivatives, alkenes and their derivatives, alkynes and derivatives, quinones, aldehydes, alcohols, ketones, phenols, ethers, anhydrides, esters, organic acids, carboxylate, carbohydrates, heterocyclic, nitriles, halogenated, aminoylates, other types; Inorganic chemical raw materials can be divided into inorganic acids, inorganic bases, inorganic salts, oxides, elemental substances, industrial gases and other types.

1. Test basis
This testing method refers to the standard of “GB/T 27807-2011 curing agent for polyester powder coatings”.
2. Detection indicators
Alcohol, ester, ketone, curing agent
3. Instrument preparation

4. Experimental conditions
Chromatographic column: 30m*0.32mm*0.25μm, DB-5 quartz capillary column
Inlet temperature: 250℃
Column temperature: initial temperature 50℃; Keep 5 min. Heating rate 30℃/min; The final temperature was 280℃ and the retention time was 7min.
FID detector temperature: 300℃
Carrier gas flow rate: 1.8mL/min

5. Atlas data

Determination of 3-Methanethiol in Sesame-Flavor Liquor

changior — Sat, 26 Mar 2022 02:26:13 +0000

3-methylthiopropyl alcohol, also known as pineapple alcohol, has a strong onion taste, dilution will appear soy sauce smell, mainly used for sauce seasoning, belongs to the edible spices allowed to use by the state. 3-methylthiopropyl alcohol was identified as the characteristic flavor substance of sesame-flavor liquor, and the appropriate detection method of 3-methylthiopropyl alcohol was very important to improve the quality of sesame-flavor liquor.

According to the national standard requirements of GB/T 20824-2007 sesame flavor liquor, the content of 3-methanethiol should be detected. In this experiment, the instrument we used was GC7900 gas chromatograph with flame photometric detector. All the gas path of the instrument is fully EPC control, all operations can be operated on the computer, simple operation, multi-point correction, correlation coefficient of 0.999 or more, in line with the national standard requirements.

1. Detection components:

3-methylthiol propyl alcohol

2.instrument configuration:

Gas chromatograph: Flame photometric detector
Chromatographic column: HT-930 30m*0.32mm*0.5μm
Standard liquid: 3-methylthiyl propyl alcohol standard liquid
Carrier gas cylinder gas: nitrogen, hydrogen, air

3. Experimental conditions

Detector: 220℃
Injector: 200℃
Column temperature: 100℃ (2min) 10℃/min to 180℃ (10min)

Application Solutions – Changior Instrument

Application of high performance liquid chromatograph in biopharmaceutics

Application of high performance liquid chromatography in the detection of drug content:

Application of high performance Liquid chromatography in the detection of pharmaceutical wastewater:

Solution: Liquor composition analysis

1.Detection Index

2.Instrument configuration

3.Experimental conditions

4.Spectra data

5.Suite of Equipment

Determination of ethylene oxide (EO) residues in medical devices

1.Detection index

2.Reagent

3.Experimental conditions

4.Spectra data

5.Instrument Configuration

Determination of total organic volatile organic compounds (TVOC) in indoor air

1.Test basis

2.Detection indicators

3.Instrument preparation

4.Experimental conditions

5.Atlas data

Automobile interior inspection scheme

1.Test basis

2.Detection indicators

3.Instrument preparation

4.Experimental conditions

5.Atlas data

Determination of solvent residue in edible vegetable oil

1. Test basis

2. Test indicators

3. Instrument preparation

4. Experimental conditions

5. Atlas data

Detection scheme of antioxidant BHA, BHT, PG, TBHQ in food

1. Test basis

2. Detection indicators

3. Instrument preparation

4. Experimental conditions

5. Atlas data

Detection protocol for 11 tetracycline antibiotics

1. Test basis

2. Detection indicators

3. Instrument preparation

4. Chromatographic conditions

Testing method for chemical solvent products

Determination of 3-Methanethiol in Sesame-Flavor Liquor

1. Detection components:

2.instrument configuration:

3. Experimental conditions

4. Configuration scheme