Blog – Changior Instrument

Detection of sucrose content using HPLC-MS analysis method

changior — Sun, 10 Sep 2023 08:51:54 +0000

Sucrose is divided into white sugar, brown sugar, soft white sugar, rock sugar and raw sugar. The molecular formula is C12H22O11, and its alias is β-D-fructofuranosyl-ah α-D-glucopyranoside, English Sucrose. It is formed by dehydration of one molecule of glucose and one molecule of fructose. It is easily soluble in water but difficult to dissolve in ethanol. Its sweetness is second only to fructose. It is an important food and sweet condiment.

In recent years, with the rapid development of high-performance liquid chromatography, this method has become a widely used detection method in the current analysis of carbohydrates. It has the advantages of high resolution, good repeatability, and high accuracy. Check out the micro-source detection laboratory Literature data used high-performance liquid chromatography to detect rice seeds.

Using Chuangjie HPLC-MS for detection. Accurately weigh 0.03g of sucrose standard and place it in a volumetric flask, add an appropriate amount of ultrapure water, and dissolve it with ultrasound to adjust the volume. Then take a 25mL volumetric flask, weigh 5.0mL of the standard stock solution, add ultrapure water, and dilute to the mark to create a mixed standard solution. All standard solutions are stored in the refrigerator and removed and returned to room temperature before use. The soluble sugar solution of fresh rice seeds was used as the sample to be tested.

The mass spectrometry conditions used electrospray ionization negative ion collection mode; capillary voltage: 3.2kV; cone voltage of 20 V, extraction voltage: 4.0V to measure sucrose, radio frequency voltage: 0.5V, source temperature: 120°C, desolvation temperature: 300℃, desolvation gas: 300L/h, cone backflush gas: 10L/h. The retention time of sucrose is 7.89 min respectively.

The HPLC-MS method is used to detect sucrose components, which is fast, simple to operate, and accurate. The sensitivity of the determination is also greatly improved, providing a method reference for accurately detecting the sugar content in medicines and foods.

Application of high performance liquid chromatograph in biopharmaceutics

changior — Tue, 26 Apr 2022 04:02:32 +0000

High performance liquid chromatography (HPLC) is an efficient and rapid separation and analysis technology developed in recent decades, and is an important tool for modern separation and detection. Highly effective liquid phase color spectrometer based on classical liquid chromatograph, introducing the theory and experiment method of gas chromatography, the mobile phase instead of high pressure conveying, adopt efficient fixed phase and on-line detection methods, development of separation and analysis techniques, with a high pressure, high speed, high efficiency, high sensitivity, high selectivity and wide scope of application, It has become one of the fastest developing and most widely used modern analytical techniques in the field of biopharmaceutical.

Application of high performance liquid chromatography in the detection of drug content:

Drugs have a great relationship with the improvement of human health and quality of life. Due to the influence of purity, the efficacy of many drugs cannot be fully played, and even produce toxic side effects. Sometimes all kinds of tests need to be done before taking, which brings a lot of trouble. The detection of high performance liquid chromatography is relatively simple, which is the best choice for separating and purifying drugs. Because high performance liquid chromatography has the characteristics of high sensitivity, rapid simplicity and specificity, widely used in drug analysis, especially in the time of more interference performance than other methods.

Application of high performance Liquid chromatography in the detection of pharmaceutical wastewater:

The environmental pollution caused by residual drug components in pharmaceutical wastewater is becoming more and more serious. The treatment of pharmaceutical wastewater has become a very important aspect of environmental protection. High performance liquid chromatograph can simultaneously determine the content of crosssalamycin, theophylline and paracetamol in pharmaceutical wastewater, through the detection of pharmaceutical wastewater, to find a suitable way to remove harmful substances in pharmaceutical wastewater.

We are a branch of Kejie holding group, in charge of the overseas marketing of Kejie products, providing to customers with chromatograph, mass spectrometry, spectrum and other analytical scientifc instruments and professional services all around the world.Welcome to visit our website: www.changior.com or contact us via email: sales@changior.com

We are looking for analytical instrument engineers worldwide

changior — Thu, 14 Apr 2022 03:19:58 +0000

Kejie holding Group is a global high-tech company integrating professional research and development, manufacturing and sales of scientific analytical instruments. Focus on environmental science, food safety, life science, inspection and quarantine, chemical energy, materials science and other industries and scientific research institutions, third-party testing institutions and government supervision and testing departments to provide a full range of laboratory equipment and overall analysis and testing solutions.

We provide analytical instruments with the latest technology in the world, provide professional solutions for scientists and laboratory analysts in GC, GCMS, LC, AAS, ICP, UV and other fields, and provide technical support in their daily testing, help researchers to do a variety of scientific analysis, assist enterprises to develop testing products. We will assist the government and other regulatory and testing institutions in providing analysis and testing services. Kejie Holdings group provides customers around the world with high-quality technical support and overall service solutions with the leading scientific analytical instruments.

At present, due to the needs of global business development, we are looking for: analytical instrument engineers to join the cooperation to serve the local market. The specific requirements are as follows:

Position: Analytical Instrument Engineer
Number of hires: 35
Job nature: full-time or part-time
Recruitment region: every country in the world

Job Responsibilities:
1. Responsible for local installation, commissioning and after-sales service of Kejie instruments;
2. Conducted technical communication with local customers on behalf of Kejie, and dealt with customers’ technical problems;
3. If the conditions and resources, can cooperate with the company to expand local related industries customer market.

Job Requirements:
1. University degree or above, major in analytical chemistry or related;
2. Have a certain understanding of analytical instruments and analytical testing market;
3. No age limit, no region limit, full-time or part-time;
4. Welcome foreign technicians and Chinese students with relevant industry background to join us.

Pay and description: who are interested in joining, can send email to contact us, and attach your resume, please send email: E-mail: service@changior.com we receive your email will be the first time to get in touch with you, negotiate specific cooperation matters.

Kejie instruments helped with the third national soil survey

changior — Thu, 31 Mar 2022 03:27:58 +0000

The State Council has issued a notice on carrying out the third national soil survey, which will start in 2022 and take four years to thoroughly identify the soil quality of agricultural land.

The census targets farmland, garden, forest, grassland and other agricultural land and some unused land soil. Among them, forest land and grassland will focus on land related to food production, while unused land will focus on land related to arable land resources, such as saline-alkali land.

Instruments and equipment are the most important part of the work involved in all the work plans of “Ten Items of soil”. Instruments and equipment are an important embodiment of the ability of soil testing laboratories, and also an effective way to ensure the smooth progress of “Ten items of soil” and improve the use efficiency of “special funds”.

Solution: Liquor composition analysis

changior — Wed, 30 Mar 2022 10:12:01 +0000

Chinese liquor culture has a long history, extensive and profound. Aroma components of liquor include alcohols, esters, acids, aldehydes and ketones, acetals, aromatic compounds, nitrogenous compounds and furan compounds, etc. Esters are aromatic compounds, which play an important role in all kinds of flavor liquor and are the main factor of forming strong aroma of liquor.

Ethyl acetate, ethyl propionate and ethyl lactate were important flavor components in liquor. The detection and control of various components in the process of liquor (flavor) production is the key to ensure the quality of liquor (flavor). More and more brand liquor production enterprises use scientific gas chromatography analysis for quality control and development of new products, and technical supervision departments at all levels use gas chromatography to monitor the quality of liquor.

1.Detection Index

Ethyl lactate, ethyl caproate, ethyl acetate, methanol, etc

2.Instrument configuration

1．Gas chromatograph with hydrogen flame ionization detector, high sensitivity
2．Chromatographic column: capillary liquor column 30m*0.32mm*0.5um
3．Standard product: 22 components of liquor standard sample
4．Chromatography workstation: double channel, a workstation can be connected to two chromatographs
5．Gas source: nitrogen cylinder gas (99.999%), hydrogen generator, air generator

3.Experimental conditions

1． Column temperature: initial temperature 50℃ initial 5min, 6℃/min, final temperature 160℃, final 10min
2． Carrier gas: nitrogen
3． Column pressure: 0.05mpa
4． Fractional injection
5． Vaporization: 230℃
6． Detector: FID 250℃

4.Spectra data

5.Suite of Equipment

Determination of ethylene oxide (EO) residues in medical devices

changior — Wed, 30 Mar 2022 09:45:27 +0000

Ethylene oxide (EO) is an organic compound with the chemical formula C2H4O. It is a known carcinogen and is often used as a sterilizer. Relevant regulations require regular testing in production and use. Ethylene oxide residue According to GB/T 16886.7-2015 biological Evaluation of Medical Devices part 7: Ethylene oxide residue sterilization GB/T 14233.1-2008 Medical infusion, blood transfusion, injection equipment test method Part 1: Chemical Analysis Methods, GB19083-2010 Technical Requirements for Medical Protective Masks, ISO10993-7; 2008 “ethylene oxide (EO) and 2-chloroethanol (ECH) residue detection” in the gas chromatography for detection.

Our company’s ethylene oxide (EO) detection solution is to quantitatively determine the content of EO in the sterilized medical equipment with headspace air chromatograph. The instrument configuration of this solution is reasonable. Simple operation is a practical testing scheme for medical equipment manufacturers.

1.Detection index

ethylene oxide (EO)

2.Reagent

Ethylene oxide (EO) standard

3.Experimental conditions

Gas phase detection conditions: column temperature 60℃, vaporization chamber 220℃, FID detector 250℃
Headspace sampling conditions: sample heating temperature 60℃, valve box temperature 100℃, pipeline temperature 120℃

4.Spectra data

Headspace injection volume was 1mL, and internal standard method was used for analysis.

5.Instrument Configuration

Determination of total organic volatile organic compounds (TVOC) in indoor air

changior — Tue, 29 Mar 2022 08:03:20 +0000

Application of Tenax TA adsorption tubes to collect a certain volume of air samples, the volatile organic compounds in air flow holds in the adsorption, after sampling, is obtained by thermal desorption device heating tube adsorption desorption gas of volatile organic compounds under test sample with an inert carrier gas into the capillary gas chromatograph, with the retention time qualitative and quantitative peak height or peak area.

1.Test basis

This protocol refers to GB 50325-2010 Appendix G “Determination of Total volatile organic Compounds (TVOC) in indoor air”.

2.Detection indicators

TVOC

3.Instrument preparation

4.Experimental conditions

Inlet temperature: 220℃
Column temperature: initial temperature 50℃, initial 5min, heating rate 6℃/min, final temperature 250℃, final 5min
Detector temperature: 250℃
Carrier gas: nitrogen, flow rate is about 30 mL /min, pre-column pressure is 0.06Mpa; Shunt sample
Combustion gas: hydrogen, flow rate of about 30 mL /min
Auxiliary gas: air, flow rate is about 300ml/m

5.Atlas data

Automobile interior inspection scheme

changior — Tue, 29 Mar 2022 07:47:44 +0000

The headspace method uses a headspace sampler and gas chromatograph. The sampling method is relatively simple, and the sampling is completed in the headspace sampler. 2 grams of samples were taken from the test sample and put into a 20ml headspace bottle, which was kept warm and balanced at 120℃ for 60min. Then a certain amount of gas was extracted from the headspace bottle with a syringe, and analyzed by gas chromatograph and FID detector.

1.Test basis

This scheme is based on THE European standard VDA277 in ISO/TS16949, and is also a common method for measuring benzene hydrocarbon volatile organic compounds in European automotive enterprises.

2.Detection indicators

Nonmetallic materials in automotive interior include benzene, toluene, xylene, ethylbenzene, styrene, formaldehyde, tetradecane, etc.

3.Instrument preparation

4.Experimental conditions

Inlet temperature: 200℃
Column temperature: initial temperature 50℃, initial temperature 5min, heating rate 12℃/min, final temperature 200℃, final temperature 3min
Detector temperature: 250℃
Carrier gas: helium, flow rate is about 30 mL /min
Combustion gas: hydrogen, flow rate of about 30 mL /min
Auxiliary gas: air, flow rate is about 300ml/min

5.Atlas data

Determination of solvent residue in edible vegetable oil

changior — Mon, 28 Mar 2022 09:04:31 +0000

Three main hexaalkanes other than n-hexane in the solvent residue of edible vegetable oil were determined by mass spectrometry. With n-hexane as the standard material, the solvent residue in edible vegetable oil was determined by headspace injection gas chromatography. The qualitative and quantitative determination methods of solvent residues in edible vegetable oil were studied.

1. Test basis

The scheme is prepared according to GB 5009.262-2016 national food safety standard Determination of solvent residues in food.

2. Test indicators

N-hexane

3. Instrument preparation

4. Experimental conditions

4.1 Headspace analysis conditions
Balance temperature: 50℃, quantitative ring temperature: 60℃, transmission temperature: 70℃, cycle time: 10min, balancing time: 30min, pressurizing time: 0.05min, quantitative ring filling time: 0.15min, quantitative ring balancing time: 0.10min, injection time: 1.00min, injection volume: 1mL.

4.2 Gas chromatographic analysis conditions
Column temperature 180℃, injector temperature: 200℃, flame ionization (FID) detector, detector temperature: 250℃, hp-5 (30m×0.25mm×0.25μm) elastic quartz capillary column, high purity nitrogen gas, flow rate of 4.4mL/min, hydrogen 50mL/min, air flow rate of 450mL/min, tail air: 15mL/min, split ratio: 1∶1.

5. Atlas data

Detection scheme of antioxidant BHA, BHT, PG, TBHQ in food

changior — Mon, 28 Mar 2022 02:16:50 +0000

Terbutylhydroquinone (TBHQ) and dibutylhydroxy toluene (BHT) are antioxidants in food additives, which can prevent or delay the oxidative decomposition and deterioration of oil or food ingredients and improve food stability. However, the addition of antioxidants must comply with the provisions of China’s food hygiene law, excessive addition will damage human health.

1. Test basis

This program is prepared according to “Determination of 9 antioxidants in Food of National Food Safety Standards”.

2. Detection indicators

Tert-butyl-p-hydroxyanisole (BHA), dibutyl hydroxytoluene (BHT), propyl gallate (PG), tert-butylhydroquinone (TBHQ).

3. Instrument preparation

4. Experimental conditions

Chromatographic column: C18 column, 4.6 mm*250 mm, particle size 5 μm
Detection wavelength: 280nm
Test temperature: room temperature
The flow speed: 1 ml/min
The flow phase: 1.5% (volume fraction) acetic acid-methanol solution (A), 1.5% (volume fraction) acetic acid-aqueous solution (B);
Elution gradient: 0min-5min, mobile phase A is 30%, 5min-20min, mobile phase A increases linearly from 30% to 80%, 20min-30min; Mobile phase A is 80%; The flow rate was 1.0mL/min and the column temperature was 40℃