Medicine Health – Changior Instrument

Application of high performance liquid chromatograph in biopharmaceutics

changior — Tue, 26 Apr 2022 04:02:32 +0000

High performance liquid chromatography (HPLC) is an efficient and rapid separation and analysis technology developed in recent decades, and is an important tool for modern separation and detection. Highly effective liquid phase color spectrometer based on classical liquid chromatograph, introducing the theory and experiment method of gas chromatography, the mobile phase instead of high pressure conveying, adopt efficient fixed phase and on-line detection methods, development of separation and analysis techniques, with a high pressure, high speed, high efficiency, high sensitivity, high selectivity and wide scope of application, It has become one of the fastest developing and most widely used modern analytical techniques in the field of biopharmaceutical.

Application of high performance liquid chromatography in the detection of drug content:

Drugs have a great relationship with the improvement of human health and quality of life. Due to the influence of purity, the efficacy of many drugs cannot be fully played, and even produce toxic side effects. Sometimes all kinds of tests need to be done before taking, which brings a lot of trouble. The detection of high performance liquid chromatography is relatively simple, which is the best choice for separating and purifying drugs. Because high performance liquid chromatography has the characteristics of high sensitivity, rapid simplicity and specificity, widely used in drug analysis, especially in the time of more interference performance than other methods.

Application of high performance Liquid chromatography in the detection of pharmaceutical wastewater:

The environmental pollution caused by residual drug components in pharmaceutical wastewater is becoming more and more serious. The treatment of pharmaceutical wastewater has become a very important aspect of environmental protection. High performance liquid chromatograph can simultaneously determine the content of crosssalamycin, theophylline and paracetamol in pharmaceutical wastewater, through the detection of pharmaceutical wastewater, to find a suitable way to remove harmful substances in pharmaceutical wastewater.

We are a branch of Kejie holding group, in charge of the overseas marketing of Kejie products, providing to customers with chromatograph, mass spectrometry, spectrum and other analytical scientifc instruments and professional services all around the world.Welcome to visit our website: www.changior.com or contact us via email: sales@changior.com

Determination of ethylene oxide (EO) residues in medical devices

changior — Wed, 30 Mar 2022 09:45:27 +0000

Ethylene oxide (EO) is an organic compound with the chemical formula C2H4O. It is a known carcinogen and is often used as a sterilizer. Relevant regulations require regular testing in production and use. Ethylene oxide residue According to GB/T 16886.7-2015 biological Evaluation of Medical Devices part 7: Ethylene oxide residue sterilization GB/T 14233.1-2008 Medical infusion, blood transfusion, injection equipment test method Part 1: Chemical Analysis Methods, GB19083-2010 Technical Requirements for Medical Protective Masks, ISO10993-7; 2008 “ethylene oxide (EO) and 2-chloroethanol (ECH) residue detection” in the gas chromatography for detection.

Our company’s ethylene oxide (EO) detection solution is to quantitatively determine the content of EO in the sterilized medical equipment with headspace air chromatograph. The instrument configuration of this solution is reasonable. Simple operation is a practical testing scheme for medical equipment manufacturers.

1.Detection index

ethylene oxide (EO)

2.Reagent

Ethylene oxide (EO) standard

3.Experimental conditions

Gas phase detection conditions: column temperature 60℃, vaporization chamber 220℃, FID detector 250℃
Headspace sampling conditions: sample heating temperature 60℃, valve box temperature 100℃, pipeline temperature 120℃

4.Spectra data

Headspace injection volume was 1mL, and internal standard method was used for analysis.

5.Instrument Configuration

Detection protocol for 11 tetracycline antibiotics

changior — Mon, 28 Mar 2022 01:53:46 +0000

Tetracyclines are a class of broad-spectrum antibiotics produced by actinomycetes. They are widely used in infections caused by various bacteria, rickettsia, chlamydia, mycoplasma, etc. In vivo, Tetracyclines complexes formed by calcium ions are yellow and deposited on bones and teeth.

Children taking Tetracyclines will have yellow teeth, and pregnant women may have discoloration of teeth and bone growth inhibition after taking Tetracyclines. Therefore, children and pregnant women should be careful with this drug or take it.

1. Test basis

This scheme is based on the standard of GB T 24800.1-2009 High performance liquid chromatography for the determination of nine tetracycline antibiotics in cosmetics.

2. Detection indicators

MINO, oxytetracycline (OTC), tetracycline (TC), differential tetracycline (ETC), aureomycin (CTC), differential aureomycin (MCTC) ), dehydrated tetracycline (ATC), dehydrated tetracycline (ETAC), doxycycline (MTC), demethylaureomycin (DMCTC), doxycycline (DC).

3. Instrument preparation

4. Chromatographic conditions

Chromatographic column: C18 column, 4.6 mm*250 mm, particle size 5 μm
Detection wavelength: 350nm
Test temperature: room temperature
The flow speed: 1 ml/min
Mobile phase: A: acetonitrile B: 0.01moL/L oxalic acid solution, gradient elution